Role and mechanism of bcl-2 and bax in high glucose-mediated apoptosis of human periodontal ligament cells

doi:10.3969/j.issn.2095-4344.2014.51.011

Abstract

Abstract:

BACKGROUND: High glucose can trigger the apoptosis of human periodontal ligament cells, in which, whether bcl-2 and bax are started? How do they work? There is still no relevant report.

OBJECTIVE: To investigate the effect of high glucose on bcl-2 and bax mRNA expression as well as apoptosis in human periodontal ligament cells in vitro.

METHODS: Human periodontal ligament cells were primarily cultured and identified. Then, cells at 5-8 passages were selected in this experiment. Cells were treated with 5.5 mmol/L (control group) and 25 mmol/L (high glucose group) glucose for 24 and 48 hours. Cell apoptosis was determined by Hoechst 33258 staining and the expression of bcl-2 and bax mRNA was detected by Real-time PCR.

RESULTS AND CONCLUSION: High glucose (25 mmol/L) could induce the apoptosis of human periodontal ligament cells and increase bcl-2 and bax significantly. The bcl-2/bax ratio showed a more significant decline in the high glucose group compared with the control group (P < 0.05). These findings indicate that high glucose can induce the apoptosis of human periodontal ligament cells, in which, Bcl-2 family plays an important role.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: periodontal ligament, diabetes mellitus, apoptosis, genes, bcl-2

CLC Number:

R318

Ren Wei-wei, Chen Shu-lan, Qiu Jing, Lu Shu-lai, Liu Hai-rong, Liu Shi-hai. Role and mechanism of bcl-2 and bax in high glucose-mediated apoptosis of human periodontal ligament cells[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(51): 8254-8260.

Figures/Tables 3

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